Maria Graziela Baldacin

Applied genetics - Prof. Dr. Schmülling Crosses of T-DNA mutants until triple homozygous obtention. Molecular techniques like DNA extraction, RNA Extraction, cDNA synthesis, PCR, qRT-PCR. Experiments with Light Induced Stress.

Management of the Sugarcane Laboratory Tech Lead of Agrobacterium Genetic Transformation (insect resistance and herbicide tolerance). Implemented a Bioreactor system increasing efficiency in 30%. Timeline coordination with green house, field trials, regulatory studies and product safety acting as a facilitator. Development of KPIs, ETS (Excellence through Stewardship), GPL( Laboratory Good Practice) documents and responsible for equipment maintenance according to the regulation.

Genetics Transformation Laboratory Technical Leader in Tissue culture development and transformation protocols. Patent information provisioning for the intellectual property department. Created an alternative method for plant genetic transformation through direct organogenesis. Responsible for Media Preparation and Microbiology Lab.

Molecular characterization of an endogenous gene of Maize using diverse methodologies, as GFP localization, in situ hybridization, overexpression of the gene using biolistic and Agrobacterium to obtain the genetically modified plants, especialization at Molecular Biology Techniques

Agrobacterium Transformation Tissue Culture protocol optimization In vitro Techniques Development Molecular Genetics Techniques such as pcr, cloning, elisa, southern, northern, western Verification of tolerance results (pathogen resistance )