Kaveri Raja

I am working on Directed differentiation of human pluripotent stem cells (hPSCs) into cardiomyocytes by canonical Wnt pathway modulation and CRISPR mediated approach. Cultivation of human pluripotent stem cells, differentiation into cardiomyocytes, cardiac organoid formation, fluorescence microscopy, immunofluorescence staining, paraffin embedding and sectioning, generation of an inducible knock-down cell line - RNA isolation, Protein isolation and Western blot

I worked on Epigenetics of Aging Project title : To investigate the functional role of CRISPR-mediated approach for targeted DNA methylation in DKK1 gene promoter on colon cancer cell lines. Techniques and skills: Bisulphite Sanger's Sequencing, DNA methylation analysis, Intestinal stem cell culture, Western Blot, Cloning, CRISPR/Cas9- Genome editing ,Molecular cloning - Transformation, Transfection, Lentivirus Transduction. Mouse and Human Organoids, Genotyping, Microscopy(Confocal and Fluorescence)